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Editorial Reviews. About the Author. Dr. Zhiping Luo is an associate professor in the ficcocaldiskpros.ml: A Practical Guide to Transmission Electron Microscopy: Fundamentals eBook: Zhiping Luo: Kindle Store. Read "Diagnostic Electron Microscopy A Practical Guide to Interpretation and Technique" by available from Rakuten Kobo. Sign up today and get $5 off your first. Read "Cellular Electron Microscopy" by available from Rakuten Kobo. Sign up today and get $5 off your first download. Recent advances in the imaging.
As fewer students and young scientists are trained in 'classical' electron microscopy, authoritative volumes such as this one become even more important and useful.
The systematic and broad approach of the chapters, especially on fixatives, dehydration and embedding provide useful data to a wide range of electron microscopists. The sections dealing with botanical specimens are especially helpful and not normally included in similar books.
The chapter on fixatives contains both theoretical and practical applications of the various different classes of fixatives. It covers a very broad range of both common and highly specific agents The discussion of organ specific fixation is very clear and a good starting point for researchers undertaking new areas of ultrastructural research.
The inclusion of topics such as stability of the polymerized resin in the electron microscope and of advanced techniques such as vacuum infiltration make this a thorough review of this area New chapters which focus on cytochemistry and cryofixation are invaluable resources especially for those just starting out in these areas. The chapter that deals specifically with low temperature embedding is very timely The culmination of this book with a chapter giving specific processing schedules is very useful Overall this is an excellent book and keeps to the high standards Audrey Glauert has set with her past volumes.
In addition, the book makes rewarding reading on its own.
The language is simple and lucid throughout, the proofreading of the text has been well nigh perfect, and the pictures potently illustrate the effects of varying crucial steps without aiming to be self-standing works of art Like the rest of the series, this book is aimed at both the novice and the expert and both should benefit from its contents. As the authors point out, the text assumes no specialised knowledge on the part of the reader; it takes you from first principles through the intricacies of technical procedures, placing most emphasis on well established methods but also showing how these are adapted for specialised requirements To summarise, this comprehensive and detailed book provides not only practical guidance, it also stimulates readers to think about what they are doing and to try new ideas.
One can only regret that it has not been available earlier. It admirably fulfills the editor's original aim of enabling the isolated worker with no specialist knowledge to pick his or her way through the bewildering array of methods available for preparing biological samples for the microscope, and to carry them out successfully In the spirit of the series this is not a book for the library shelf but will be an invaluable companion at the laboratory bench Overall the book is well organized, clearly written and crammed with easily accessible information.
The text is both well referenced and amply illustrated with high quality micrographs, photographs of equipment, tables and some elegant line drawings Sufficient theoretical information is included to provide a sound understanding of the principles, yet above all this is a book for the practising microscopist struggling with problems at the bench.
It will become an essential acquisition for all microscopy preparation laboratories and of great value both to beginners and more experienced users wishing to keep up with contemporary specimen preparation technology. Young, Proceedings of the Royal Microscopical Society.
It is, in fact, much more than an updated edition, because it has been extensively revised and significantly extended to include many new techniques. The new book follows a logical sequence through the initial fixation of samples to the various options of embedding for TEM It is a much needed back-to-basics text, and is an excellent introduction for new microscopists. It is also an excellent source for the expert microscopist to consult and to reassess his "routine" methodology.
I certainly found myself questioning how certain dogma had crept into our own lab practices after reading this text.
This book brings together many of the loose threads from other books on specimen preparation that address specific points but never quite succeed in providing a comprehensive overview of biological specimen preparation for TEM. In short, this book will provide a sound basis for the discussion and choice of methods of biological specimen preparation for TEM for the next 25years. Skepper, BioEssays. The experienced microscopist will read with interest all the detailed descriptions of embedding media chemical composition and properties, polymerization reactions and chemical hazards.
Glauert was one of the developers of the basic embedding techniques and therefore has invaluable experience, together with strong opinions, on the value of the published procedures This useful basic book can help newcomers to biological electron microscopy learn about the evolution of techniques since the early days of the field 50 years ago, and to use state-of-the-art procedures to prepare samples.
Almost all you could wish to know about fixation, dehydration, and embedding is in this book, making it a useful asset. It contains enough theory for the user to understand why procedures are performed the way they are, and the procedures themselves are fully explained, with a wealth of practical tips.
It really is an essential work for beginners, for instruction and reference, but experienced users may find it a helpful compendium of recent developments. I would recommend this book to beginners and experienced microscopists alike and have no doubt that it will be a book that is referred to frequently in any laboratory. The first few books in the series were already well-established when I was an undergraduate.
Who can forget the seminal volume 3, Fixation, Dehydration and Embedding of Biological Specimens , or volume 10, Low Temperature Methods in Biological Electron Microscopy , a particular favourite of mine.
Now, some 25 years on, we have reached volume It also covers major system components, including light sources, After reviewing the main Knowledge of microscope design is rapidly becoming more important. Microscopes are used in critical applications such as drug development, clinical tests, and genomics.
Considerable expertise is required for the evaluation, design, and manufacture of these instruments.
Several subsystems must be integrated: Microscopes are used in critical Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope-skills such as how to align microscope optics and perform image processing.
Fundamentals of Light Microscopy and Electronic Imaging explores the Over the last decade, advances in science and technology have profoundly changed the face of light This established, popular textbook provides a stimulating and comprehensive introduction to the insects, the animals that represent over half of the planet's biological diversity.
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